Side effects detection of drug candidate on microarray

Project: Research

Project Details

Description

This project have aimed to developpe a screening tool for side effect detection of drug candidate, potentially toxic.
Using microarrays, RNA samples from any cell or tissue type can be analysed for changes in transcript levels. Such changes are characteristic of developmental processes, cancer, infection, response to drugs, virtually all biological events are accompanies by specific changes in RNA transcription.

The Biochip have been designed to efficiently address toxicologically-relevant gene pathways including : DNA damage, oxidative stress, cell signaling, cell proliferation, DNA repair, detoxification metabolism, transcription apoptosis, inflammation. Specially designed for toxicologists, this microarray offers the opportunity to identify chemicals with a potential toxic effect and to better understand how chemicals can cause toxicity. It also gives the possibility to study how these chemicals alter the structure and the function of a cell. It will also be used to better adress a number of key toxicology issues, including mode of action, dose response relationships and chemical interactions.

First, gene expression profiles regulated by known toxic compounds were analysed in order to create a databank of expression pattern.

By the analysis of differentially expressed genes, toxic molecules will be detected pretty early in the development process and they will allow their rapid elimination in the new drugs discovery process.
AcronymHepatochips
StatusFinished
Effective start/end date1/02/0031/01/03

Keywords

  • gene expression
  • DNA microarray
  • toxicology