Transcription factors activity is traditionally measured by gel retardation. Although this technique is sensitive, it is radioactive, difficult to adapt for automatisation and is not suited for screening. We have developped an alternative method for measuring DNA-binding capacity of transcription factors which presents many advantages : it is non-radioactive (colorimetric), rapid, more sensitive than gel retardation, and suits large-scale screening. The method is now working for NF-kappaB, CREB, AP-1, HIF and PPAR.
|Effective start/end date||1/10/00 → 31/12/01|
- colorimetric assay
- Transcription factors
- Gel retardation