Identification of proteins interacting with a regulatory DNA sequence depends on in silico analysis for putative transcription factors binding sites, a process generating a lot of false positive and false negative results. We develop a new method to isolate nuclear proteins that physically bind to a DNA sequence, synthesized by PCR, and to identify them by mass spectrometry
|Effective start/end date||1/09/05 → 31/01/13|
- transcription factor purification
- DNA affinity
- transcription factor identification
- mass spectrometry
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