Development of a method for identification by mass spectrometry of proteins interacting with a oligonucleotidic sequence

Project: PHD

Project Details

Description

Identification of proteins interacting with a regulatory DNA sequence depends on in silico analysis for putative transcription factors binding sites, a process generating a lot of false positive and false negative results. We develop a new method to isolate nuclear proteins that physically bind to a DNA sequence, synthesized by PCR, and to identify them by mass spectrometry
StatusFinished
Effective start/end date1/09/0531/01/13

Keywords

  • transcription factor purification
  • DNA affinity
  • transcription factor identification
  • mass spectrometry