Comparative structural study of type 1 and 2 isopentenyl diphosphate isomerases (IDI)

We would like to understand the catalytic mechanisms of the isoformes 1 and 2 of the isopentenyl diphosphate isomerases, key enzyme in the biosynthesis, in particular, of the steroïds. Enzyme IDI-1 is present in mammalians, plants or yeasts, and is essential in their development. Enzyme IDI-2 is vital for pathogenic microorganisms. The catalytic mechanism of IDI-1 and that of IDI-2 will be characterized by various complementary techniques of structural chemistry.